Qiagen
Cignal NF-kB reporter (luc) Kit
CCS-013L
The goal was to evaluate levels of transcription induced by NF-kB transcription cofactors. The purpose of the research is to observe effects of drugs/agents on such transcription levels. This will help evaluate specificity of certain drugs for certain signaling pathways. This reporter assay kit was chosen because it is the only known luciferase-tagged (since a luminometer was present in our lab) reporter available on the market.
Reporter Assays
Human breast cancer cells
Transfect cultured cells using lipofectamine, incubated for 24-48 hrs prior to detection on luminometer
Must optimize number of cells cultured to ensure efficient transfection
Relative luciferase activity is calculated as quotient of firefly and renilla luciferase (for normalization)
N/A
Easy to use
None
NF-kB reporter assay can detect transcription and can be used to evaluate drug effects