Superior Conjugated ST2 Antibody for Flow Cytometry

Cancer Immunology and Virology
Dana-Farber Cancer Institute
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

MD Biosciences

Product Name:

T1/ST2 (IL-33R) Monoclonal Antibody, FITC

Catalog Number:

101001F

Single cell suspension of mouse lung cells was stained with surface markers including lineage markers (CD3, NK1.1, CD11c, etc), CD45, CD90.2, ST2. I have tried multiple fluorochrome-conjugated ST2 antibodies from different vendors. I found this antibody to be far superior as compared to the others.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

1:300, 30 mins, 4 C

Blocking Agent

Mouse Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Blue laser on a BD Fortessa

Results Summary

Approximately, 1/3rd of the lineage negative cells in the mouse lung were found to express ST2 receptor.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works much better than other available ST2 antibodies on the market.

The Bad

Expensive, only available in two fluorochrome conjugations.

The Bottom Line

Definitely recommended for separating ST2+ cells from ST2- cells.

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