Biolegend
AF488 anti-mouse F4/80
123120
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Browse Similar Products: F4/80 Flow Cytometry Antibodies
Our lab studies the effect of influenza infection on the function of immune cells in different cohorts of mice. We need a good F4/80 antibody to isolate the macrophages and study their function during and after infection. This antibody allows us to visualize this large population without too much spillover.
Flow Cytometry
Mouse spleen cells
Cells were stained in 100uL FACS buffer with antibody at 1:200; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
None
LSR II
By staining our mouse spleen cells, we are able to use this antibody to find F4/80 cells in our cell isolations for downstream analysis.
N/A
Not too bright
This is a good antibody for visualizing large F4/80+ cell populations in mice tissue.