Sigma
3,3′-Dioctadecyloxacarbocyanine perchlorate (Dio)
34215-57-1
The green fluorescent, 3,3′-Dioctadecyloxacarbocyanine perchlorate (Dio) is highly fluorescent and quite photostable when incorporated into membranes. In our Dio stained cell viability study, the fluorescent of Dio were stable for detection for over 48 hours incubation.
Cell viability Assay
Dog blood
Primary cells from blood were incubated with Dio for 15min in dark. Cell were then washed twice to removed extra dye in PBS.
Incubation in dark
In our Dio stained cell viability study, the fluorescent of Dio were stable for detection for over 48 hours incubation. After indicated time points, Dio stained cells were then incubated with propidium iodide. The cell viability were detected by flow cytometry.
N/A
Stable for long time incubation
Not found
Stable for long time incubation.