Cell Signaling Technology
Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199)
4228
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I used this antibody for a signalling pathway study and to see whether there is some factor activating pi3k or not.
Western Blot
Caco2 cell lysate
1:1000 dilution, overnight at 4 degrees
5% Bovine serum albumin dissolved in 1%TBST
1:5000 dilution in 1x TBST, 1hr, room temperature
None
ECL substrate used developed by G-BOX chemiluminiscent
I got results from this antibody but they are not good. It gives multiple bands and high background.
N/A
Bands will come
Multiple bands and high background
This antibody has two molecular weights. It gives multiple bands and a high background.