V5 Anti Peroxidase Antibody

August 03, 2017

chemical biology
indian institute of chemical technology

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Company:

Sigma

Product Name:

Monoclonal Anti-V5-Peroxidase antibody

Catalog Number:

V2260

Browse Similar Products: V5- Peroxidase Western Blot Antibodies

We cloned the all mitiotic assembling checkpoint proteins in v5 tag containing vector. To understand the cross talk between the proteins, we are using pull down assays and knock down experiments.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Hela cell lysate

Primary Incubation

1:1000 dilution, at room temperature for 2hr

Blocking Agent

Skimmed milk

Secondary Incubation

Not required

Tertiary Incubation

Not required

Detection

ECL solution

Results Summary

We have got clear bands of V5 tag with a protein of interest using this antibody.The antibody has not given any nonspecific bands.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good

The Bad

No negative remarks

The Bottom Line

Recommended for use in probing the V5 tagged fusion proteins.

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