Rockland Immunochemicals, Inc.
MOUSE IgG (H&L) Antibody Peroxidase Conjugated
610-1302
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I used this secondary antibody for western blotting. It was good working antibody. It gives clear bands without background.
Western Blot
Caco2 cell lysate
1:1000 dilution, overnight at 4 degrees
BSA dissolved in 1x TBST
1:4000 dilution in 1x TBST, 1hr, room temperature
None
ECL substrate was used and then developed by G-BOX chemiluminiscent
Many secondary antibodies gives more background, but this antibody gives clear bands without background.
N/A
No background
Nothing
It was a good working antibody