Isotype Control For PE Labeled Antibodies

August 08, 2017

Pharmaceutical Technology
CIBER BBN
Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE Rat IgG2b, κ Isotype Ctrl Antibody

Catalog Number:

400607

We use this antibody as an isotype control for IgG2b, k antibodies labeled with PE dye. It helps to discern unspecific signal from specific labeling.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single cell suspension from mouse spleen

Primary Incubation

1:100

Blocking Agent

We block with CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

PE channel in the flow cytometer

Results Summary

It discerns very well between nonspecific signal and specific signal

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Really good price

The Bad

None

The Bottom Line

I recommend its use as isotype control

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