Isotype Control For FITC Labeled Antibodies

August 07, 2017

Pharmaceutical Technology
CIBER BBN
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Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC Rat IgG2b, κ Isotype Ctrl Antibody

Catalog Number:

400605

We use this antibody as an isotype control for IgG2b, k antibodies labeled with FITC dye. It helps to discern unspecific signal from specific labeling.

 

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single cell suspension from mouse spleen

Primary Incubation

1:100

Blocking Agent

We use CD16/32

Secondary Incubation

None

Tertiary Incubation

Labeled Antibodies

Detection

We detect in the channel for FITC in the flow cytometer

Results Summary

It is very helpful to discern nonspecific signal of specific signal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good price

The Bad

None

The Bottom Line

I recommend because the rate accuracy/price

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