Vector Laboratories
VECTOR Hematoxylin QS
H-3404
Info:Supplier Page View Product Specs View Product
We study the expression of a newly discovered receptor, transcobalamin receptor (TCblR), required for uptake of cobalamin by cells. We studied TCblR expression in adult mouse kidneys where we used hematoxylin qs as a counterstain.
Immunohistochemistry
Paraffin embedded mouse kidney sections
After DAB staining, sections were washed in PBS and then incubated with hematoxylin qs for 5 minutes. The sections were then washed in tap water, twice, to remove excess hematoxylin, dehydrated in 70% to 100% ethanol, cleared in xylenes and mounted with permount.
Incubation needs to be at least 5 minutes for paraffin sections else the stain goes away when dehydrated in ethanol afterwards.
We found TCblR expression in glomerulus in kidneys cortex region.
28351841
N/A
Gives a good contrast
Gives a background if incubated for long
It works really well on paraffin embedded sections and is very easy to use. It does not require any dilution and can be stored at room temperature.