R&D Systems
Recombinant Rat CCL2/JE/MCP-1 protein, CF
3144-JE-025/CF
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Rat aortic smooth muscle cells (RASMCs) were treated with MCP-1 at 50 ng/ml concentration and the migration of RASMCs were measured as a percentage of wound closure.
Cell migration assay
Rat aortic smooth muscle cells
RASMCs were seeded in an IBIDI chambers and after 1-2 days when the cells are nearly confluent, cells were quiesced and before treating with rat MCP-1, the inserts were removed and the cell migration was measured after 24 hrs and the measured as a percent of wound closure.
The effects may be very minimal if the cells are old. I got good results when I use the cells just between 3-5 passages after retrieving the cells from liq. N2.
rat MCP-1 induces RASMC migration
N/A
Good agonist to study cell migration mechanisms.
Needs optimization with the concentrations and the time.
Very good chemotactic agent to understand the mechanisms of cell migration and the cell signaling pathways.