Sigma-Aldrich
Monoclonal anti-beta actin antibody-peroxidase conjugate
A3854
Browse Similar Products: beta actin Western Blot Antibodies
Beta actin is one of the most used loading (normalization) control in Western blotting experiments. Sigma's HRP conjugated beta-actin antibody makes the normalization step a quick one without the need for stripping the membrane.
Western Blot
Human coronary artery endothelial cell lysates
1:40,000 dilution in 3% non-fat skim milk for 2 hours at RT.
3% non-fat skim milk for 30 min (PVDF), 1 hour (nitrocellulose)
Not required
NA
Chemi luminescence
20 ug endothelial cell lysate was used in this experiment. After SDS-PAGE and blotting, the membrane was blocked with 3% non-fat skim milk and incubated with 1:40,000 diluted anti-beta actin-HRP antibody.
If you are reusing the blot, no need of stripping and reprobing. Block the membrane again after first use and incubate with beta-actin HRP antibody.
Very strong signals and working dilution 1:40000
None
Beta-actin-HRP conjugate enables one step normalization of Western blots.