Iba1 Polyclonal Antibody for Microglia/Macrophage Staining

July 11, 2017

Cell Biology and Molecular Genetics
University of Maryland, College Park
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Wako Chemicals USA, Inc.

Product Name:

Anti Iba1 Rabbit

Catalog Number:

019-19741

One of the goals of our lab is to study neuroinflammation and involves examining various cell types including microglia/macrophages. We have routinely stained for Iba1 which is a marker for this cell population.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse spinal cord paraffin-embedded sections

Primary Incubation

Anti-Iba1

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

Biotin-conjugated goat anti-rabbit

Tertiary Incubation

DAB

Detection

Light microscopy

Results Summary

Microglia/macrophages are readily seen in inflamed tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Staining can be easily optimized and microglia/macrophages are readily seen

The Bad

Not a good antibody if you want to distinguish between microglia and macrophages

The Bottom Line

I would recommend this antibody to anyone who wants to stain for microglia/macrophages.

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