Sigma-Aldrich
Anti-a-actinin antibody
A7732
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Anti-a-actinin monoclonal antibody from Sigma is the best antibody for marking cardiomyocytes in immunofluorescence staining. It also works very well for Western blotting.
Immunofluorescence
FFPE sections of mouse heart
1:500 dilution, overnight at 4C in 3% BSA prepared in 1x PBS
5% donkey serum in 3% BSA
2 hours at RT in 3% BSA prepared in 1x PBS
None
Mouse heart FFPE sections were permeabilized with 0.1% Triton-X100, blocked and probed with anti-a-actinin antibody (1:500) followed by Alexa Fluor 488 conjugated secondary antibody. Sections were counter stained with isolectin-AF647 and Hoechst 33342.
N/A
High specificity and low background, suitable for both immunofluorescence and western blot.
Well suited antibody to mark cardiomyocytes in immunofluorescence