ZO-1 Immunofluorescence Staining

June 27, 2017

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

ZO-1 Antibody (R40.76)

Catalog Number:

sc-33725

The aim is to stain ZO-1 in human gingival epithelial cells using immunofluorescence staining. We could not get positive staining in either frozen sections or paraffin embedded sections.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human gingival tissue

Primary Incubation

4C overnight, 1/50-800 dilution

Blocking Agent

10% goat serum with 1%BSA in PBS

Secondary Incubation

Goat anti rat Alexa Fluor 488

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

We could not get positive staining in either frozen sections (acetone or formalin fixation) or paraffin embedded sections (with or without antigen retrieval).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

None so far

The Bad

No positive staining & high background

The Bottom Line

We have tried many different ways to make it work, unfortunately, all efforts failed. I recommend their JAM-A antibody (sc-53623) for tight junction staining which works in both frozen and paraffin embedded sections.

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