Anti-CD28 Antibody For In Vitro Costimulation Of CD4+ T Cells

June 29, 2017

Cell Biology and Molecular Genetics
University of Maryland, College Park
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Purified anti-mouse CD28 Antibody

Catalog Number:

102101

Our lab studies CD4+ T cells and often performs in vitro or ex vivo (re)activation using anti-CD3 + anti-CD28.

Experimental Design and Results Summary

Applications

In vitro activation of CD4+ T cells

Sample

Murine CD4+ T cells

Primary Incubation

Goat anti-hamster IgG coated plates

Blocking Agent

None

Secondary Incubation

Anti-CD3 + anti-CD28

Tertiary Incubation

None

Detection

ELISA for cytokines to assess T cell activation

Results Summary

We coat plates with goat anti-hamster IgG for anti-CD3 crosslinking. Cells are stimulated for 24 hours. Supernatants are collected and cytokines are measured by ELISA to assess T cell activation. We see robust activation of T cells using this method and these antibodies.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable and reproducible results

The Bad

None

The Bottom Line

Great antibody for T cell costimulation. Reliable and reproducible results. Also, a relatively inexpensive antibody.

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