Biolegend
Purified anti-mouse CD28 Antibody
102101
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Our lab studies CD4+ T cells and often performs in vitro or ex vivo (re)activation using anti-CD3 + anti-CD28.
In vitro activation of CD4+ T cells
Murine CD4+ T cells
Goat anti-hamster IgG coated plates
None
Anti-CD3 + anti-CD28
ELISA for cytokines to assess T cell activation
We coat plates with goat anti-hamster IgG for anti-CD3 crosslinking. Cells are stimulated for 24 hours. Supernatants are collected and cytokines are measured by ELISA to assess T cell activation. We see robust activation of T cells using this method and these antibodies.
N/A
Reliable and reproducible results
Great antibody for T cell costimulation. Reliable and reproducible results. Also, a relatively inexpensive antibody.