Western Blot Stripping Buffer

University of Maryland, College Park
Cell Biology and Molecular Genetics
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Restore™ PLUS Western Blot Stripping Buffer

Catalog Number:

46430

Western blots are routinely performed in our lab and often require probing with multiple primaries. This often requires us to strip the blots before coming in with additional antibodies. This stripping buffer is easy to use and does not take much time to perform a decent strip.

Experimental Design and Results Summary

Application

Western blot stripping

Starting Material

Western blot of murine protein

Protocol Overview

Our lab performs strips with this buffer as follows:-Seal blot in plastic bag/container and seal -Place in 48*C water bath for 30minutes-Wash blot and reblock

Tips

Be sure that blot is completely covered with stripping buffer to ensure even stripping

Results Summary

Blots with no signal from previous primaries and secondaries

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

I would recommend this product to anyone needing an easy and reliable stripping buffer for western blots.

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