Detection Of Murine EZH2- T Lymphocytes

Biochemistry and Molecular Biology
University of Arkansas for Medical Sciences
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Ezh2 (D2C9) XP Rabbit mAb

Catalog Number:

5246

Using the EZH2 (62C9) antibody from Cell Signaling Technology, I was able to detect a clear band at the predicted molecular weight. These results were clean and reproducible.

Experimental Design and Results Summary

Applications

Western Blot

Sample

T-lymphocyte whole murine cell extract

Primary Incubation

1:1000 for 1 hour at RT

Blocking Agent

5% milk in PBST

Secondary Incubation

1:3000 Rabbit secondary HRP

Tertiary Incubation

None

Detection

ECL

Results Summary

Detected distinct band at the predicted size of 98 kDA. Very clean and reproducible. During lymphocyte activation, there is an increase in expression of EZH2.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clean and reproducible

The Bad

None

The Bottom Line

Use this antibody to detect murine EZH2.

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