Vertical Electrophoresis

CIBER BBN
Pharmaceutical Technology
Researcher

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Criterion™ Cell

Catalog Number:

1656001

The system allows vertical electrophoresis to run one or two precast protein gels or homemade midi gels. This system requires only 1L running buffer and the slots location helps to easily and quickly slide cassettes into position making the electrophoresis of proteins much easier.

Experimental Design and Results Summary

Application

Protein electrophoresis

Starting Material

Protein extract from a HEK cell line

Protocol Overview

With two precast Gel or home made gels, you can run up to 26 samples in less than 1 hr. It easy to load the samples after mixing them with loading buffer.

Tips

None

Results Summary

Proteins are really well separated after one hour and you just need to choose the appropiate gel to perform the separation. We use precast gels and they work very well. Next, gel can be stained or transfer into a membrane for western blot.

Features Summary

None

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to be use

The Bad

None

The Bottom Line

Once you use with the precast gels you do not want to go back to traditional protein electrophoresis

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