Tonbo Biosciences
redFluor 710 Anti-Human CD45RA (HI100)
80-0458
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In this experiment, human PBMCs were analyzed for transcription factor expression across CD4 and CD8+ T cell subsets. A large multi-color panel was required, such that we wanted to include a third color for the red laser. Tonbo Bioscience's redFluor 710 was selected as an alternative to Alexafluor 700 and similar reagents. We stained with CD45RA-redFluor 710 to differentiate naive and memory lymphocytes. This antibody provided excellent resolution of CD45RA expression levels in CD8+ gated T cells, actually enabling us to see 3 different levels of expression high, intermediate, and low.
Flow Cytometry
Human PBMCs
1:75; 30 minutes; on ice; FACS buffer
2% FBS, 2mM EDTA, in PBS
None
BD Fortessa
The CD45RA-redFluor 710 antibody fit well in a complex multi-color panel involving human lymphocytes and retained good resolution of 3 levels of CD45RA expression in CD8+ T cells. This antibody outperformed other antibodies that we have tried which only showed 2 distinct levels of expression. Notably, the fluorescence intensity and resolution was preserved with aldehyde fixation and subsequently permeablization.
N/A
excellent resolution of CD45RA expression; enables 3 color analysis with the red laser
Excellent choice for multi-color panels involving human T cells. Budget-priced by Tonbo Biosciences.