Consistent Results with CD45.2 Antibody

May 23, 2017

GIVI
The Gladstone Institutes
Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

FITC Mouse Anti-Mouse CD45.2

Catalog Number:

553772

Our lab aims to understand T-cell mediated immunity in the genital mucosa and the gut. The purpose of using this antibody was to determine the presence of CD45.2 congenic markers vs CD45.a to prepare for future investigations and breeding.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse PBMCs

Primary Incubation

4 C for 30 minutes at 3:300 dilution

Blocking Agent

FC block (1:1000)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Results had indicated successful detection of 2 populations containing CD45.2 markers in our combined samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very reliable antibody and has always yielded consistent results

The Bad

Rather pricey

The Bottom Line

Dependable antibody that provides expected results.

Share your experience with other scientists. Write a Review! »

Join the discussion