Miltenyi Biotec
Pan T Cell Isolation Kit, human
130-096-535
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Love this kit. It's easy, fast, and efficient. Routinely get >95% purity of CD3+ cells from buffy coat-derived PBMCs. I can highly recommend this kit.
T cell purification
PBMCs from human buffy coats
Ficoll-based PBMC extraction.Red blood cell lysis using Ammonium Chloride Solution.Spin down desired number of starting PBMCs and resuspend in 400ul MACS buffer.Add 100ul Pan T Cell Biotin-Antibody Cocktail and mix. Incubate 5min at 4°C.Add 300ul MACS buffer and 200ul Pan T Cell MicroBead Cocktail and mix.Incubate 10min at 4°C.Set up column on MACS magnet and equilibrate with 3ml MACS buffer.Add cells to column and collect flow-through (negative selection kit).Wash column with 5ml MACS buffer and collect flow-through.
Follow the protocol and you'll be fine
From 100 million PBMCs, yields typically range from 20-40 million cells with viabilities and purities around 90% or higher.
Isolation of a pure and untouched T cell population
N/A
Easy, fast, and efficient
A bit on the expensive side
Great product. Would highly recommend.