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PerCP/Cy5.5 anti-human CD183 (CXCR3) Antibody
353714
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CD183 or CXCR3 is a Gαi protein-coupled chemokine receptor and is generally related to leukocyte migration and trafficking when expressed on Th1 cells. It may also be expressed upon T effectors as reports suggest. However a population of CD8+ T cells that express CD183 has recently been shown to function like T regulatory cells. Along with CD183, these cells also express CCR7 and CD45RA and stain positively for Foxp3.
Flow Cytometry
Human PBMC or peripheral blood
PBMC’s (stimulated vs. non-stimulated) were stained with 1ul (1:100) of anti-CD183 Percpcy5.5 antibody (the same cells were simultaneously stained for a multi-color panel with CD8 PE, CD4 FITC, CCR7 APCcy7, CD45RA PEcy7 antibodies according to the experiment design). Cells were kept for 30 mins. at room temperature. After incubation cells were washed twice with 1XPBS and re-suspended in 500ul of staining buffer. Cells were then analyzed by flow cytometry where mononuclear cells were gated according to FSC and SSC and expression of CD183 cells on CD4 or CD8 T cells was analyzed.
N/A
Flow cytometric detection for Percpcy5.5 channel
A distinct population of CD8+ CD183+ cells were observed in patient samples. On further analysis of this population (CD8+CD183+) almost all cells expressed CD45RA and CCR7. They were foxp3 positive and thus marked a population termed as CD8 Tregs.
Good Clear detection of positive cell population
Nothing to mention
CD183 Percpcy5.5 anti-human antibody gives excellent detectable results by flow cytometry using human PBMC's.