The Antibody Stains B220+ B Cells Very Well

May 10, 2017

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Alexa Fluor® 488 anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103225

We wanted to study the B cells in healthy vs diseased states, so we used this antibody to identify B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse lymph node cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody identifies B220+ B cells very well.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody stains B220+ cells very well

The Bad

None to mention

The Bottom Line

The antibody is good for identifying B cells.

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