Anti-Mouse OX40L

May 04, 2017

Biology
Abbvie
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

anti-mouse OX40L biotin

Catalog Number:

559798

The purpose was to determine the basal level of expression of OX40L on BALB/c spleen. The antibody was chosen due to the availability on this fluorochrome.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1ug per test, 30min on ice

Blocking Agent

Fc block 5 minutes on ice

Secondary Incubation

Streptavidin conjugated BV650 antibody, 15 minutes on ice

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

The results show no expression of OX40L on myeloid cells. I expected to see higher expression. Need to confirm findings with another antibody clone to confirm.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Low to undetectable staining

The Bottom Line

Unclear if the antibody is good or not at this point

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