eBioscience™ from Invitrogen Antibodies
CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), PE
12-0711-82
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Browse Similar Products: CD71 Flow Cytometry Antibodies
We used CD71 and Ter119 staining to look at RBC development in the bone marrow based on the gating strategy previously published in 2011 by Koulnis et.al. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3211121/). This CD71 antibody conjugated to PE worked very well.
Flow Cytometry
Bone marrow from OT-II mouse, which is CD4 TCR transgenic mouse specific for OVA peptide presented on I-Ab
+4C 30 mins
Anti-CD16/32
None
LSR II
Great resolution between CD71 positive and negative cells. We were able to identify 4 distinct populations of RBC and its progenitors based on their CD71 + Ter119 staining and further discriminating these by FSC (size).
N/A
Bright, good resolution
Definitely a keeper.