Excellent Antibody For Olig2

pediatrics
University of Mississippi Medical Center
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

EMD Millipore

Product Name:

Olig2

Catalog Number:

AB9610

Browse Similar Products: Olig2 Immunohistochemistry Antibodies

Tested for rat brain tissue as well as oligodendrocyte cell culture. Very good staining. Need pre-treatment with higher concentration of triton (~0.5-0.8% in PBS) because the antigen is located in the nucleus.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Free-floating sections of rat brain

Primary Incubation

Anti-Olig2 1:800 dilution, overnight, RT

Blocking Agent

10% goat serum with 0.5% triton in PBS

Secondary Incubation

Rb-IgG-Alexa 555 (1:1000), RT, 2 h

Tertiary Incubation

None

Detection

Red fluorescence alexa 555

Results Summary

Bright labeling with low background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright staining with low background. Consistent results.

The Bad

None

The Bottom Line

The best Olig2 antibody

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