Anti Mouse CD45.1 Antibody For Flow Cytometry

April 28, 2017

Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

Anti-Mouse CD45.1 FITC

Catalog Number:

11-0453

The general aim of my research is to study acute myeloid leukemia. We are transplanting human leukemic cells into immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice and monitoring leukemic progression.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow isolated from NSG mice transplanted with human leukemic cells

Primary Incubation

1:100

Blocking Agent

Fc block is added to cells prior to staining with antibody

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD LSRII-yellow machine

Results Summary

This antibody produces a reliable and bright signal by flow cytometry. It provides good separation from the human CD45 antibody, allowing detection of mouse versus human cells in bone marrow of transplanted mice to easily be measured.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable and reproducible data

The Bad

None

The Bottom Line

Recommended for flow cytometry

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