Anti-Mouse CD80

May 04, 2017

Biology
Abbvie
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PerCP-Cy™5.5 Hamster Anti-Mouse CD80

Catalog Number:

560526

The general aim is to determine the level of CD80 after maturation of myeloid cells in the mouse. This antibody was chosen because of the fluorochrome availability.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

30 min on ice using manufacturer's recommended concentration

Blocking Agent

Fc block for 10 minutes on ice

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

The results did not show robust staining of cells. This was unexpected. May have to try a different clone.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Some staining

The Bad

Not robust staining

The Bottom Line

Should try a different clone to see if this lack of staining is real.

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