Biolegend
Rat IgG2a, kappa APC
400511
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This antibody was used as an isotype control for another antibody. The goal of the experiments is to determine non specific binding. This product was selected because of availability.
Flow Cytometry
Mouse splenocytes
30minutes on ice
Fc Block 10 minutes on ice
None
The titration of this antibody shows that this antibody does not bind nonspecifically to the cells, which is what I wanted out of the isotype.
N/A
Good isotype
Need to titrate to confirm it's a good isotype
Good isotype for the purpose