Fixable Viability Dye

April 24, 2017

Abbvie
Biology
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Fixable Viability Dye UV

Catalog Number:

65-0868-14

The purpose of using this dye is to stain for viability and to use the UV channel on the cytometer. This antibody was chosen because it is prediluted in DMSO and the protocol is simple to follow.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Mouse Cells

Protocol Overview

Cells were washed with PBS and then incubated with 1:500 of the dye for 30minutes at 4C. The cells were then washed in facs buffer.

Tips

Optimize the concentration

Results Summary

The results suggest there is some separation between dead and live cells. This separation is not as wide as the product sheet suggests.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Concentration used produced consistent results

The Bad

Needs to be optimized for your experiments

The Bottom Line

The concentration of reagent needs to be optimized for your cell type. But I use a standard 1:500 dilution for all cell types which seems to be okay.

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