Goat Anti-Hamster Secondary Antibody, Alexa Fluor 488

Pathology
Albert Einstein College of Medicine
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen by Thermo Fisher Scientific

Product Name:

Goat anti-Hamster IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Catalog Number:

A-21110

Our lab uses various antibodies generated in hamsters. This antibody was used as a secondary antibody for the detection of hamster immunoglobulins by fluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain

Primary Incubation

Primary was incubated overnight at 4 degrees celcius

Blocking Agent

2% Milk and goat serum

Secondary Incubation

1ug/ml was used for 1 hour at room temperature

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

Cells were easily detected by immunofluorescence microscopy with very little background

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use and very sensitive

The Bad

None

The Bottom Line

Great Alexa Fluor 488 antibody.

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