Great GFAP Antibody

Pathology
Albert Einstein College of Medicine
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

EMD Millipore

Product Name:

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5

Catalog Number:

MAB3402

We analyse brain sections and use various antibodies to determine differences in cell types and numbers between different mouse models of multiple sclerosis. We use this antibody to detect mouse differentiated astrocytes by immunohistochemistry (paraffin).

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse brain

Primary Incubation

Overnight

Blocking Agent

2% Milk and goat serum

Secondary Incubation

Anti-Mouse

Tertiary Incubation

None

Detection

DAB

Results Summary

We were able to detect the presence of astrocytes in all of our stained sections using 5ug/ml.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Results are consistent

The Bad

None

The Bottom Line

This antibody is perfect for distinguishing astrocytes from other glial cells.

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