Invitrogen Antibodies by Thermo Fisher Scientific
CD4 Monoclonal Antibody (RM4-5), Super Bright 600, eBioscience™
63-0042-80
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Browse Similar Products: CD4 Flow Cytometry Antibodies
We were given CD4 Super Bright 600 to try by our local eBioscience sales rep. The Super Bright dyes are eBiosciences's answer to the Brilliant violet dyes sold by BD Bioscience and Biolegend. Here I present titrations on CD4 SB 600 and my review of this antibody.
Flow Cytometry
Murine Splenocytes
30 mins @ +4C
Anti-CD16/32
None
LSR II
I included two other CD4 antibodies as comparisons, CD4 APC and CD4 Alexa 700. While the CD4 SB 600 (1:400) gave a very bright positive population, there was quite a bit of background staining as seen from the spread of the negative population. This spread is most likely intrinsic to the SB600 dye as the other fluorochromes were also conjugated to the same antibody clone (RM4-5).
N/A
Very bright. Minimal spillover into BV510 and BV421 channels.
Some background staining.
Probably not my first choice for my go-to CD4 antibody.