Anti-Mouse IFNgamma APC Antibody For Intracellular Flow Cytometric Analysis Of Murine T Cells

Cell Biology and Molecular Genetics
University of Maryland, College Park
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

APC Rat Anti-Mouse IFN-γ Clone XMG1.2 (RUO)

Catalog Number:

562018

One ongoing project going on in the lab is studying CD4+ T cells in vitro and in in vivo mouse models. This requires a robust and reliable set of surface and intracellular markers for immune cell populations. The IFNgamma antibody is a great antibody when examining CD4+ T cells for Th cell populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

In vitro differentiated murine Th1 cells

Primary Incubation

Anti-CD3 and anti-CD4 surface staining

Blocking Agent

None

Secondary Incubation

APC Rat Anti-Mouse IFN-γ Clone XMG1.2 (RUO)

Tertiary Incubation

Additional intracellular stains if desired

Detection

LSR-II Flow Cytometer

Results Summary

IFNgamma is readily stained and with levels of Th1 cells expressing this antigen present comparable to those reported in the literature.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stains desired antigen when used at optimal dilution

The Bad

None

The Bottom Line

Definitely a suitable antibody for use with staining murine Th1 CD4+ T cells

Share your experience with other scientists. Write a Review! »

Join the discussion