Heat-Inactivated M. Tuberculosis For In Vivo Mouse Experiments

Albert Einstein College of Medicine
Pathology
PhD Candidate

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Company:

BD Biosciences

Product Name:

BD Difco™ M. Tuberculosis H37 Ra, Desiccated, 100 mg. Killed M. tuberculosis H37 Ra for use in adjuvants (6/sp)

Catalog Number:

231141

On of the main in vivo models used in our lab is the EAE model which requires immunization of genetically susceptible mice with myelin oligodendrocyte glycoprotein (MOG). In order to successfully elicit an immune response, MOG peptide with complete Freunds adjuvant (CFA) to create an emulsion. This is then injected into mice.

Experimental Design and Results Summary

Application

In vivo injections in mice (EAE model)

Starting Material

Heat-inactivated M tuberculosis and incomplete Freuds adjuvant

Protocol Overview

Combine M tuberculosis with incomplete Freunds adjuvant and thoroughly grind/mix both for ~10 minutes in a mortar and pestle to make complete Freunds adjuvant.

Tips

Use a mortar and pestle to grind M Tb into the incomplete adjuvant

Results Summary

Mice injected with MOG + complete Freunds adjuvant emulsion develop EAE

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Works very well in murine EAE

The Bad

None

The Bottom Line

Good product for in vivo use, especially the EAE model

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