B220 Staining Of Spleen Cells

Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

Anti-Hu/Mo CD45R (B220) APC

Catalog Number:

47-0452-82

The aim of the research was to study germinal center B cells. The goal for the experiment was to measure the levels of different receptors and compare them between non-germinal center and germinal center B cells from immunized mice. I chose this product because of the price and it fight my flow cytometry panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:100, 4 degrees, 1 hour

Blocking Agent

2% FBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

Clear identification of B cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, clearly marks positive cells

The Bad

None

The Bottom Line

Good antibody for B cell staining

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