The Good
Directly labelled with a fluorophore. Already titrated by the manufacturer before shipping so very little optimization is required.
The Bottom Line
I highly recommend this antibody for use in flow cytometry to measure TIM-3 expression on human blood cells. Given the relatively low, or lack of expression on resting/non-stimulated T-cells as observed here, you may want to have a positive staining control when measuring TIM-3 expression on biological samples to ensure that you are getting adequate staining as observed with our PMA/Ionomycin-stimulated PBMC.