IgG Isotype Control

March 28, 2017

Veterinary Diagnostic Laboratory
Kansas State University
RA

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Goat Polyclonal IgG Isotype Control

Catalog Number:

NB410-28088F

This isotype control was used to differentiate between red blood cells that bind to IgM, IgA and C3 in dog species.It worked well as a negative control antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood in EDTA from dog

Primary Incubation

20 mins in dark with antibody

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS Calibur

Results Summary

Works well as a negative control

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great Antibody

The Bad

N/A

The Bottom Line

Will use again.

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