Histology Stain For Neutral Lipids

March 27, 2017

Albert Einstein College of Medicine
Pathology
PhD Candidate

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Company:

Poly Scientific R&D Corp.

Product Name:

Oil Red O for Lipids

Catalog Number:

cy011

A common practice in our lab is to stain mouse brain and spinal cord sections for the presence of neutral lipids following inflammation induced by different induction methods including experimental autoimmune encephalomyelitis (EAE) and cuprizone toxicity. The presence of neutral lipids indicates the incomplete clearance of myelin debris. The Oil Red O stain provides a qualitative means of visualizing debris clearance.

Experimental Design and Results Summary

Application

Qualitative immunohistochemical evaluation of lipids

Starting Material

Frozen coronal mouse brain sections

Protocol Overview

Rehydrate frozen tissue in ddH2O for 2 minutes, incubate sections in absolute propylene glycol for 2 minutes, incubate sections in Oil Red O for 48 hours, incubate section in 85% propylene glycol for 1 minute, rinse in ddH2O twice, stain with hemotoxylin, coverslip

Tips

Filter Oil Red O before staining to avoid precipitate during staining, determine the optimal incubation time for your tissues and experimental conditions

Results Summary

Stained slides present with distinctive reddish-purple droplets.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Gives great staining of lipids as distinctive droplets when lipid debris is being cleared

The Bad

Hard to distinguish between extracellular debris and intracellular lipids

The Bottom Line

Good stain to examine qualitative lipid debris following inflammation

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