Staining Of Murine Oligodendrocytes In Frozen Histology Sections

Pathology
Albert Einstein College of Medicine
PhD Candidate

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Quality of Results

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Company:

EMD Millipore

Product Name:

Anti-Olig-2 Antibody

Catalog Number:

AB9610

Browse Similar Products: Olig-2 Immunohistochemistry Antibodies

Our lab studies the role of receptor tyrosine kinases in demyelination/remyelination and neuroinflammation. To examine the presence of total oligodendrocytes in sections during remyelination, we stain frozen brain sections with anti-Olig2 antibody and quantify.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Frozen coronal sections of mouse brain

Primary Incubation

Anti-Olig-2 Antibody at 1:200

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

Goat anti-mouse IgG2b Alex-Fluor 588 1:1000

Tertiary Incubation

DAPI

Detection

Light microscopy

Results Summary

Olig2 is a transcription factor and can be readily visualized in the nucleus. We can easily visualize oligodendrocytes using this antibody with immunofluorescence.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great antibody for staining total oligodendrocytes and easily to visualize when sections are also stained with DAPI

The Bad

None

The Bottom Line

If staining mouse sections for OLs, use this antibody.

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