Compensation Beads To Use With Antibodies Raised In Mice

Albert Einstein College of Medicine
Microbiology & Immunology
PhD Candidate

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Company:

BD Biosciences

Product Name:

Anti-Mouse Ig, κ/Negative Control Compensation Particles Set

Catalog Number:

552843

In our lab we do many flow cytometry experiments with human cells for which we use panels of mouse anti-human antibodies. Since we stain the same sample with many different antibodies we need to set up compensation controls to use in the set up of the cytometer in order to be able to distinguish the differently stained populations of cells

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Compensation Beads

Protocol Overview

mix a drop of positive and a drop of negative control beads in a FACS tube. Stain with single antibody (same staining protocol as used when staining cells). Set up compensation controls on the cytometer before collecting any cell samples

Tips

Can prepare the compensation controls in advance or use them for several experiments in the future.

Results Summary

Easily distinguished positive and negative control. Very helpful for FACS experiments where several colors are used in a single sample

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to use, reliable

The Bad

None

The Bottom Line

Would recommend this product

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