Vector Laboratories
Neurobiotin Tracer
SP-1120
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My research explores the function and circuity of the retina in health and disease. Specifically, I'm interested in determining how synaptic transmission is altered at early staged of glaucoma. A accomplish this principally using electrophysiological patch-clamp recordings in mouse retina. Neurobiotin tracer allows me to obtain high-quality images of the cells I recorded from to confirm neuronal identity and analyze neuronal structure. Include in patch pipette or inject using a sharp microelectrode. A classic reagent, works great.
Neuroanatomy
Mouse retina
Include in patch pipette solution at 1%, dye diffuses into cell during recording. Or, use depolarizing current steps to drive neurobiotin in.
Follow included directions
Great. Clear labeling of processes
N/A
Classic tool, works well.
Need to incubate with streptavidin to visualize after fixation and processing
Classic reagent, works well for single neuron anatomy.
Product Name: NEUROBIOTIN™ TracerSupplier Page