Cell Signaling Technology
Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb
4147
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Browse Similar Products: Cleaved Notch1 Western Blot Antibodies
Notch is synthesized as an inactive form which is proteolytically cleaved to release the intracellular domain (NICD) from the membrane. This antibody detects the activated intracellular domain of notch1 after cleavage (NICD)
Western Blot
HUVEC lysate
1:2000, overnight
5% milk
1:5000, 1 hour
NA
ChemiDoc™ Touch Imaging System
Clean, very bright band were detected at the predicted size
Can be used at a lower concentration than 1:2000
Clean, bright bands
Overnight incubation
Great antibody for western blot detection of NICD