Great Fluorescent Secondary Antibody For Western Blots

March 13, 2017

Urology
UCSF
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitorgen ThermoFisher Scientific

Product Name:

Goat anti-Mouse IgG (H&L) Secondary Antibody, DyLight 800 4X PEG

Catalog Number:

SA5-35521

We use these fluorescent secondary antibodies for western blots with the licor odyssey. They give good signal and allow you to image across a range of intensities.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse

Primary Incubation

Actin 1:2000, overnight at 4 degree

Blocking Agent

Odyssey blocking buffer

Secondary Incubation

1:10,000 2 hrs room temp

Tertiary Incubation

None

Detection

Licor

Results Summary

Good signal with very little background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well, fluorescent allows you to image over a range of intensities compared to traditional film

The Bad

None

The Bottom Line

I would recommend using these thermo fluorescent antibodies as secondaries for western blots.

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