Anti-CD69 Identifies Activated T-Cells In The Lymph Node

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 510™ anti-mouse CD69 Antibody

Catalog Number:

104532

We wanted to examine how T-cells in the lymph nodes are affected in a healthy vs diseased state. To do this, we wanted to look at their activation, and therefore stained for CD69

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse lymph node cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody clearly identifies CD69+ cells in the CD4 T-cell population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Antibody identifies CD69+ cells well

The Bad

None to mention

The Bottom Line

The antibody is good for identifying activated T-cells

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