Biolegend
anti-mouse CD4 FITC
100405
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This antibody was used to phenotype T cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD4, FITC works well with other fluorophores in the staining panel, and Biolegend had a great price.
Flow Cytometry
Mouse splenocytes
1:100 dilution in 100uL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
CD4 staining was as expected. Positive cells were fully resolved from negative.
Works well, low cost.
Will continue to use this antibody.