Anti-human CD107a (LAMP-1) Antibody Review

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-human CD107a (LAMP-1) Antibody

Catalog Number:

328616

We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by activating them via TCR specific MHC-peptide ligands, and co-stimulatory molecules. We use purified CD8 T cells from naive donors with HLA types, and stimulate them in vitro to then look at their degranulation and cytokine profile. The data shown here represents results from a positive control, where CD8 T cells were activated with PMA and ionomycin and then stained different cytokine production.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Purified CD8+ T Cells from human PBMC

Primary Incubation

1.5 ul in 200 ul total volume of media, as cells incubated in 37 degrees Celcius for 6 hours

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Good staining for cell degranulation. Staining during incubation is a must.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works great.

The Bad

None

The Bottom Line

I recommend this antibody

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