EasySep Mouse CD4+ T Cell Isolation Kit

February 13, 2017

University of Montana
DBS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

STEMCELL Technologies

Product Name:

EasySep Mouse CD4+ T Cell Isolation Kit

Catalog Number:

19852A

We use this kit to isolate untouched CD4+ T cells from murine spleens for downstream culturing.

Experimental Design and Results Summary

Application

Cell Isolation

Starting Material

Murine Single Cell Suspension

Protocol Overview

Can perform up to 10e8 cells per magnet separation. Follow manufacturer's protocol for this 20 minute cell separation.

Tips

I double the time stated for all incubation steps.

Results Summary

This product purifies murine CD4+ cells from spleens at greater than 95% purity.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to use and effective.

The Bad

None

The Bottom Line

I recommend this kit for isolation of untouched mouse CD4+ T cells.

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