K9 IP Experiment

Veterinary Diagnostic Laboratory
Kansas State University
RA

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Company:

Biolegend

Product Name:

Alexa Fluor® 700 anti-mouse/human CD11b

Catalog Number:

101222

The aim of this experiment is to detect presence of T or B cell lymphoma in dogs. The antibody CD11b is used in gating the granulocytic population in whole blood cells in the dogs. The antibody used worked very well for doing so.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Dog whole blood in EDTA

Primary Incubation

30 mins in dark

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FAS CAlibur

Results Summary

The antibody in some case shows the presence of granulocytes.

DOI or PMID #

NA

Additional Notes

This antibody cross reacts with canine species.

Related Categories

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Summary

The Good

Serves as a good antibody for detection of granulocytes.

The Bad

None.

The Bottom Line

Will recommend.

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